If you have found a technology of interest, you can make your own selection for further information
|
|
|
|
|
|
| Method to obtain intact, viable protoplasts from grape berry mesocarp cells and biotechnological applications | | | Inventors |  |
| | | Hernâni Gerós e - Departamento de Biologia | | Natacha Fontes - Departamento de Biologia | | Serge Delrot - Institute of Vine and Wine Sciences (ISVV), University Victor Segalen - Departamento de Biologia |
| | Abstract | |
| The University of Minho and University Victor Segalen developed a method for protoplast isolation from grape berry mesocarp cells which is fast, efficient and low-cost. It allows the attainment of highly pure intact and viable protoplasts, involving the treatment of mesocarp tissue with highly pure enzyme preparations that do not affect protoplast viability.
This method allows the development of plants capable of surviving in inhospitable conditions.
| | Description | |
| Grapes (Vitis spp.) are economically the most important fruit species in the world. In 2005, vineyard area and grape production was of 7,488,196 ha and 66,901,419 t, respectively, ranking second in fruit production (FAO 2007). The development and maturation of grape berries have received considerable scientific scrutiny because of both the uniqueness of such processes to plant biology and the importance of these fruits as a significant component of the human diet and wine industry. The present method relates to the biotechnology of plants and in particular to techniques aiming the investigation, improvement or modification of plants which entail the use of plant protoplasts. The use of isolated protoplasts for plant genetic manipulations to improve crop plants is an increasingly important aspect of plant biotechnology. Isolated protoplasts from grape berry mesocarp cells, may be subjected to genetic manipulation
(transformation with vectors, protoplast fusion to obtain hybrids) and then cultured to regenerate whole plants. Protoplasts are an ideal system for DNA delivery and plants derived from protoplasts are generally clonal in origin. This provides a useful tool for any transformation system, because
it will eliminate chimerism in transgenic plants. Also, the in vitro culture of protoplasts in a large-scale may be useful to produce secondary metabolites like resveratrol, among others. Additionally, protoplasts can be used as a biological model to understand virus infection of higher plants by following the complete replication cycle of the virus. Physiological, biochemical and molecular studies in cultured protoplasts can provide important information on the understanding and control of fungal plagues. Resistant plants to certain fungal species involved on diseases such as eutypiose may be produced based on protoplast technology.
| | Industrial Aplication | |
| Agronomical, biotechnological, and pharmaceutical industries
| | Stage of Development | |
| Development phase - laboratory tested
| | Industrial Property Status | |
| Patent applied for but not yet granted
| | Collaboration Sought | |
| Partner for RTD project
License agreement
Technical Co-operation
Joint venture agreement
Manufacturing agreement (Subcontracting & Co-contracting)
Commercial Agreement with Technical Assistance
| | Reference Number | |
| TMPT103851
|
|
|
|
|
|
|
|
|
|
|
|
|
